منابع مشابه
Mice cloned from embryonic stem cells.
Cloning allows the asexual reproduction of selected individuals such that the offspring have an essentially identical nuclear genome. Cloning by nuclear transfer thus far has been reported only with freshly isolated cells and cells from primary cultures. We previously reported a method of cloning mice from adult somatic cells after nuclear transfer by microinjection. Here, we apply this method ...
متن کاملNon-equivalence of cloned and clonal mice
Cloning mice by nuclear transfer is conventionally performed using a one-step procedure. Recently an alternative two-step procedure has been proposed for generating mice that are clonally derived from a single somatic donor nucleus. Here, we demonstrate that such mice may not be clones of the original donor nucleus. In the conventional one-step cloning by nuclear transfer (nt), a donor cell nuc...
متن کاملproduction of cloned mice by nuclear transfer of cumulus cells
background: over the past several years, mammals have been successfully cloned by either the splitting of an early stage embryo or nuclear transfer of adult somatic cells (nt) into oocytes. although it has been 15 years since the generation of the first cloned mammals from somatic cells by nt, the success rate for producing live offspring by this technique is low regardless of the cell type and...
متن کاملP-86: Production of Cloned Mice by Somaticm Cell Nuclear Transfer
Background: For several years, mammalian cloning by splitting an early embryo or nuclear transfer into oocytes method has been successfully performed. Cloning is now also possible using adult somatic cells. Although it has now been 15 years since the first cloned mammals were generated from somatic cells using nuclear transfer (NT), the success rate for producing live offspring by cloning is lo...
متن کاملMice cloned from induced pluripotent stem cells (iPSCs).
Differentiated somatic cells of various species can be reprogrammed into induced pluripotent stem cells (iPSCs) by ectopically expressing a combination of several transcription factors that are highly enriched in embryonic stem cells (ESCs). The generation of iPSCs in large animals has raised the possibility of producing genetically modified large animals through the nuclear transplantation app...
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ژورنال
عنوان ژورنال: Genome Biology
سال: 2000
ISSN: 1465-6906
DOI: 10.1186/gb-spotlight-20000925-03